NAD+ subcutaneous injection has emerged as the most practical high-bioavailability delivery method for NAD+ repletion outside of a clinical IV setting. This guide covers the complete protocol from vial receipt to injection.
Reconstitution
NAD+ is typically supplied as a lyophilized powder in 500mg vials. Add 5mL of bacteriostatic water to a 500mg vial = 100mg/mL concentration. For a 100mg dose, draw 1mL. For a 50mg dose, draw 0.5mL. Always inject BAC water slowly down the side of the vial and gently swirl — NAD+ is sensitive to vigorous agitation.
Dosing Protocol
| Phase | Dose | Frequency | Duration |
|---|---|---|---|
| Loading | 100mg | Daily | 2–4 weeks |
| Maintenance | 50–100mg | 3–5x/week | Ongoing |
Injection Technique
Use a 29–31 gauge, 0.5-inch insulin syringe. Pinch the skin at the injection site (abdomen or thigh preferred), insert at a 45-degree angle, aspirate briefly, then inject slowly. NAD+ can cause a mild burning sensation at the injection site — this is normal and resolves within minutes. Rotating sites reduces local irritation.
Storage
Unreconstituted: stable at room temperature for 3 months, indefinitely at -20°C. Reconstituted: refrigerate at 2–8°C, use within 4 weeks. Keep away from direct light. NAD+ is more light-sensitive than most peptides — store in the original amber vial or wrap in foil.